| | Klubb | Tränare | Liga | Arena | Hemmaort | Grundad |
 | Forte Taganrog | Eduard Sarkisov | Russian Second League Division A | Forte Arena | Taganrog | 2020 |
| Dynamo Stavropol | Ashamaz Shakov | | Dynamo Stadium Stavropol | Stavropol | 1933 |
| Avangard Kursk | Igor Belyaev | Russian Second League Division A | Trudovye Rezervy Stadium | Kursk | 1946 |
| FC Murom | Aleksandr Kulchiy | Russian Second League Division A | Andrey-Arena | Murom | 2014 |
| Dynamo Vladivostok | | Russian Second League Division A | Dynamo Stadium | Vladivostok | 1944 |
| Rodina-2 Moskva | Aleksandr Laktionov | Russian Second League Division A | Spartakovets Stadium | Moscow | 2022 |
| FC Kaluga | Evgeniy Losev | Russian Second League Division A | Annenki Arena | Kaluga | 2002 |
| Dynamo Kirov | | Russian Second League Division A | Dinamo Kirov | Kirov | 1923 |
| SKA Rostov | | Russian Media Football League | SKA SKVO | Rostov-on-Don | 1937 |
| FC 2DROTS | | Russian Media Football League | Arena-Khimki | Moscow | 2016 |
| Amkar Perm | Andrey Blazhko | | Zvezda | Perm | 1993 |
| Broke Boys | | Russian Media Football League | Arena CSKA | Moscow | 2022 |
| Znamya Truda | | | Znamya Truda | Orekhovo-Zuyevo | 1909 |
| Saturn | | | Saturn | Ramenskoe | 1933 |
| FC Amkal | | Russian Media Football League | Sportivniy Gorodok | Moscow | 2018 |
| Dynamo-2 Moskva | | Russian Second League Division A | VTB Arena | Moscow | 1986 |
| Dinamo Vologda | Rudolf Chesalov | Russian Second League Division B | Dinamo | Vologda | 1927 |
| Zenit-2 St. Petersburg | | Russian Second League Division B | Petrovskiy | Saint Petersburg | 1993 |
| FC Astrakhan | | Russian Second League Division B | Imeni Kolosova | Astrakhan | 1931 |
| Rodina Media Moskva | | Russian Media Football League | Stadion Spartak | Moskva | 2023 |
| Salyut Belgorod | Viktor Navochenko | | Salyut | Belgorod | 1923 |
| Kosmos Dolgoprudnyi | | | Salyut Stadium | Dolgoprudny | 1998 |
| FC Chertanovo | Sergey Chikishev | | Yantar | Moscow | 1993 |
| Spartak Tambov | | | Spartak Stadium | Tambov | 1960 |
| Nart Cherkessk | Aleksey Zhdanov | Russian Second League Division B | Nart | Cherkessk | 1982 |
| FC Kuban Holding | | | Spartakovets Stadium | Pávlovskaya | 2015 |
| FC Tver | | | Znamya Truda | Tver | 1957 |
| FC Krasnodar-2 | Ilya Valiev | | Krasnodar Academy Stadium | Krasnodar | 2013 |
| Volna Kovernino | Oleg Makeev | Russian Second League Division B | Zavolzhskiy | Kovernino | 2016 |
| Torpedo Vladimir | Denis Evsikov | Russian Second League Division B | Torpedo Stadium (Vladimir) | Vladimir | 1959 |
| Uralets-TS | Igor Bakhtin | Russian Second League Division B | Uralvagonzavod | Nizhny Tagil | 1946 |
| Biolog Novokubansk | | | Biolog Progress | Novokubansky District | 1995 |
| FC Baltika-2 Kaliningrad | | | Baltika | Kaliningrad | 2021 |
| Nosta Novotroitsk | | | Metallurg | Novotroitsk | 1991 |
| Sokol Kazan | | Russian Second League Division B | Sokol | Kazan | 2018 |
| Krasnoye Znamya Noginsk | | | Istomkino Stadium | Noginsk | 1911 |
| Legion Dynamo Makhachkala | | | Stadion Dynamo | Makhachkala | 2015 |
| Sever Murmansk | | | Tsentralnyi Profsoyuz Stadion | Murmansk | 1961 |
| FK Irkutsk | Konstantin Dzutsev | Russian Second League Division B | Trud | Irkutsk | 2019 |
| FK Druzhba Maikop | | | Olimp-2 | Maikop | 1981 |
| FK Ryazan | | | CSK Stadium | Ryazan | 2010 |
| Khimki-M | | Russian Second League Division B | Novye Khimki | Khimki | 1997 |
| Spartak-2 Moskva | | Russian Second League Division B | Spartakovets imeni N.P. Starostina | Moscow | 1964 |
| FC Rostov-2 | | | Rostov Arena | Rostov-on-Don | 1992 |
| Zvezda Perm | | | Zvezda | Perm | 1932 |
| Spartak Nalchik | Timur Shipshev | | Republican Spartak | Nalchik | 1959 |
| Peresvet Domodedovo | | | Avangard Stadium | Domodedovo | 2016 |
| FC Kolomna | | Russian Second League Division B | Avangard Stadium | Kolomna | 1906 |
| FC Orenburg-2 | | | Gazovik Stadium | Orenburg | 2001 |
| Dynamo Barnaul | | | Stadion Dinamo | Barnaul | 1957 |
