| | Klubb | Tränare | Liga | Arena | Hemmaort | Grundad |
 | Ventura County FC | | USL Championship | Dignity Health Sports Park | Los Angeles | 2014 |
| Portland Timbers 2 | Cameron Knowles | | Merlo Field | Portland | 2015 |
| Milwaukee Torrent | | National Premier Soccer League | Hart Park | Milwaukee | 2015 |
| Real Monarchs SLC | Jámison Olave | USL Championship | Rio Tinto Stadium | Sandy | 2014 |
| FC Miami City | | | Central Broward Park | Miami | 2014 |
| Virginia Cavalry FC | | | Edelman Financial Field | Ashburn, Virginia | 2012 |
| FC Cincinnati 2 | Tyrone Marshall | MLS Next Pro | NKU Soccer Stadium | Cincinnati | 2021 |
| Denton Diablos | | National Premier Soccer League | Mean Green Soccer Stadium | Denton, Texas | 2018 |
| Houston Dynamo 2 | | MLS Next Pro | SaberCats Stadium | Houston | 2021 |
| Phoenix FC | | | Sun Devil Soccer Stadium | Phoenix | 2012 |
| VSI Tampa Bay | | | Plant City Stadium | Plant City | 2011 |
| Colorado Rapids 2 | | MLS Next Pro | Denver Soccer Stadium | Denver | 2021 |
| Tacoma Defiance | | USL Championship | Cheney Stadium | Seattle | 2014 |
| Huntsville City | | MLS Next Pro | Joe W. Davis Stadium | Huntsville | 2022 |
| Reno 1868 FC | Ian Russell | | Greater Nevada Field | Reno | 2015 |
| Capo FC | | NISA | Belson Stadium | San Juan Capistrano, California | 2006 |
| Irvine Zeta | | NISA | Highmark Stadium | Irvine, California | 2022 |
| New York RB 2 | Ibrahim Sekagya | USL Championship | Columbia Soccer Stadium | New York | 2015 |
| Orlando City B | Marcelo Neveleff | | Titan Soccer Complex | Melbourne | 2015 |
| Atlanta United 2 | Steve Cooke | USL Championship | Coolray Field | Lawrenceville | 2017 |
| North Texas SC | | USL League One | Toyota Stadium | Frisco | 2018 |
| New England Revolution II | | USL League One | Gillette Stadium | Boston | 2019 |
| Queensboro FC | | USL Championship | York College | New York City | 2019 |
| Fort Worth Vaqueros FC | | National Premier Soccer League | Farrington Field | Fort Worth, Texas | 2013 |
| Corpus Christi FC | | | Jack Dugan Family Soccer and Track Stadium | Corpus Christi | 2017 |
| Austin FC II | | MLS Next Pro | Parmer Field | Austin | 2022 |
| Crown Legacy FC | | MLS Next Pro | Sportsplex at Matthews | Matthews | 2022 |
| Chicago Fire II | | MLS Next Pro | SeatGeek Stadium | Bridgeview | 2021 |
| Columbus Crew 2 | Federico Higuaín | MLS Next Pro | Historic Crew Stadium | Columbus | 2021 |
| Los Angeles FC 2 | | MLS Next Pro | Titan Stadium | Los Angeles | 2022 |
| Minnesota United 2 | Jeremy Hall | MLS Next Pro | Allianz Field | Minnesota | 2021 |
| New York City FC II | | MLS Next Pro | Belson Stadium | New York City | 2021 |
| The Town FC | | MLS Next Pro | PayPal Park | San Jose | 2021 |
| St. Louis City SC 2 | | MLS Next Pro | CityPark | St. Louis | 2021 |
| Santa Barbara Sky | | USL League One | Titan Stadium | Santa Barbara, California | 2020 |
| Brooklyn FC | | USL League One | Future Legends Complex | Brooklyn, New York | 2023 |
| Seacoast United Phantoms | | | New England Sports Park | Hampton, New Hampshire | 1996 |
