| | Klubb | Tränare | Liga | Arena | Hemmaort | Grundad |
 | Westchester SC | | USL League One | The Stadium at Memorial Field | Westchester County | 2024 |
| Charlotte Independence | Mike Jeffries | USL Championship | American Legion Memorial Stadium | Charlotte | 2014 |
| AV Alta FC | | USL League One | The Hangar | Lancaster | 2023 |
| Spokane Velocity FC | | USL League One | Skyline Sports Complex | Spokane, Washington | 2021 |
| Pittsburgh Riverhounds | Bob Lilley | USL Championship | Highmark Stadium | Pittsburgh | 1999 |
| Forward Madison | Matt Glaeser | USL League One | Breese Stevens Field | Madison | 2018 |
| Portland Hearts of Pine | | USL League One | Fitzpatrick Stadium | Portland | 2023 |
| Loudoun United | Ryan Martin | USL Championship | Loudoun United Stadium | Leesburg | 2018 |
| Chattanooga FC | | | Finley Stadium | Chattanooga | 2009 |
| Tormenta FC | Ian Cameron | USL League One | Erk Russell Park | Statesboro | 2015 |
| Greenville Triumph SC | | USL League One | Legacy Early College | Greenville | 2018 |
| FC Naples | | USL League One | Paradise Coast Sports Complex Stadium | Naples | 2024 |
| One Knoxville SC | Ian Fuller | USL League One | Highmark Stadium | Knoxville, Tennessee | 2021 |
| Chattanooga Red Wolves | | USL League One | David Stanton Field | Chattanooga | 2018 |
| Central Valley Fuego | | USL League One | Parmer Field | Fresno, California | 2020 |
| Memphis 901 | | USL Championship | AutoZone Park | Memphis | 2018 |
| Texoma FC | Adrian Forbes | USL League One | Torero Stadium | Sherman, Texas | 2023 |
| Richmond Kickers | Darren Sawatzky | USL League One | City Stadium | Richmond | 1993 |
| Austin Bold | | USL Championship | Bold Stadium | Austin | 2017 |
| Miami United | | National Premier Soccer League | Flamingo Park | Miami | 2012 |
| RGV FC Toros | | USL Championship | RGVFC Stadium | Edinburg | 2015 |
| Carolina Core FC | Donovan Ricketts | MLS Next Pro | Truist Point | High Point | 2022 |
| FC Baltimore Christos | | National Premier Soccer League | Marriotts Ridge High School | Marriottsville, Maryland | 2018 |
| Club de Lyon | | NISA | Daytona Stadium | Orlando | 2021 |
| Los Angeles Force | Dekel Keinan | NISA | Veterans Memorial Stadium | Long Beach | 2019 |
| Real Monarchs SLC | Mark Lowry | USL Championship | Rio Tinto Stadium | Sandy | 2014 |
| Northern Colorado Hailstorm | | USL League One | Future Legends Complex | Windsor, Colorado | 2021 |
| Portland Timbers 2 | Cameron Knowles | | Merlo Field | Portland | 2015 |
| FC Tucson | | USL League One | Kino Sports Complex | Tucson | 2010 |
| Houston Dynamo 2 | | MLS Next Pro | SaberCats Stadium | Houston | 2021 |
| St. Louis City SC 2 | | MLS Next Pro | CityPark | St. Louis | 2021 |
| San Diego Loyal | | USL Championship | Torero Stadium | San Diego | 2019 |
| Inter Miami CF II | Cristian Ledesma | USL League One | DRV PNK Stadium | Miami | 2019 |
| OKC Energy FC | | USL Championship | Pribil Stadium | Oklahoma City | 2013 |
| Ventura County FC | Matthew Taylor | USL Championship | Dignity Health Sports Park | Los Angeles | 2014 |
| Columbus Crew 2 | Federico Higuaín | MLS Next Pro | Historic Crew Stadium | Columbus | 2021 |
| Huntsville City | | MLS Next Pro | Joe W. Davis Stadium | Huntsville | 2022 |
| New York Cosmos | Dane Murphy | National Premier Soccer League | James M. Shuart Stadium | New York | 1971 |
| Orlando City B | Manuel Goldberg | | Titan Soccer Complex | Melbourne | 2015 |
| New England Revolution II | | USL League One | Gillette Stadium | Boston | 2019 |
| Capo FC | | NISA | Belson Stadium | San Juan Capistrano, California | 2006 |
| Irvine Zeta | | NISA | Highmark Stadium | Irvine, California | 2022 |
| Atlanta SC | Steve Guppy | | Atlanta Silverbacks Park | Atlanta | 1995 |
| Rochester Rhinos | | | Sahlen's Stadium | Rochester | 1996 |
| Virginia Cavalry FC | | | Edelman Financial Field | Ashburn, Virginia | 2012 |
| Tacoma Defiance | | USL Championship | Cheney Stadium | Seattle | 2014 |
| Chicago Fire II | | MLS Next Pro | SeatGeek Stadium | Bridgeview | 2021 |
| Los Angeles FC 2 | Junior González | MLS Next Pro | Titan Stadium | Los Angeles | 2022 |
| New York City FC II | | MLS Next Pro | Belson Stadium | New York City | 2021 |
| FC Miami City | | | Central Broward Park | Miami | 2014 |
